Article
In vitro mutagenesis of a xylanase from the extreme thermophile Caldocellum saccharolyticum.
Applied microbiology and biotechnology - 1 Jan 1992
Lüthi E, Reif K, Jasmat N B, Bergquist P L
Abstract excerpt
Six mutant xylanases were obtained by in vitro mutagenesis of a xylanase gene from the extremely thermophilic bacterium Caldocellum saccharolyticum. The temperature stability of all enzymes was affected by mutation to various degrees and one of the xylanases had an altered temperature optimum. The mutations had no effect on the pH optimum. The C. saccharolyticum xylanase showed strong homology to several...
Topics
- Amino Acid Sequence
- Bacteria, Anaerobic
- Enzyme Stability
- Escherichia coli
- Glycoside Hydrolases
- Hot Temperature
- In Vitro Techniques
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Sequence Homology, Nucleic Acid