Temperature and induction effects on the degradation rate of an abnormal beta-galactosidase in Escherichia coli.

doi:10.1016/0168-1656(91)90235-n

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Article

Temperature and induction effects on the degradation rate of an abnormal beta-galactosidase in Escherichia coli.

Journal of biotechnology - 1 Apr 1991

Kosinski M J, Bailey J E

Abstract excerpt

Intracellular protein degradation was investigated using an unstable fragment of Escherichia coli beta-galactosidase, the CSH11 mutant, as a model protein. This abnormal protein was expressed from a single copy gene in the chromosome and is converted to a detectable degradable intermediate. The i...

Topics

Escherichia coli
Kinetics
Mutation
Temperature
beta-Galactosidase

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