Article
Gene typing of Chlamydia trachomatis by polymerase chain reaction and restriction endonuclease digestion.
Sexually transmitted diseases - 1 Jan 2000
Gaydos C A, Bobo L, Welsh L, Hook E W, Viscidi R, Quinn T C
Abstract excerpt
A portion of the major outer membrane protein (MOMP) gene from 15 Chlamydia trachomatis serovars was amplified by polymerase chain reaction (PCR) and the product was analyzed by restriction fragment length polymorphism (RFLP). A set of primers was used to amplify an 871 base pair gene fragment encompassing the 4 hypervariable regions of MOMP. AluI digestion of the product gave distinctive patterns for the 15...
Topics
- Bacterial Outer Membrane Proteins
- Base Sequence
- Blotting, Southern
- Chlamydia trachomatis
- Genes, Bacterial
- Genotype
- Molecular Sequence Data
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length