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Article

Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp.

Journal of clinical microbiology - 1 May 1992

Kaltenboeck B, Kousoulas K G, Storz J

Abstract excerpt

Specific and sensitive amplification of major outer membrane protein (MOMP) gene (ompA) DNA sequences of Chlamydia species with various MOMP genotypes was achieved by a two-step polymerase chain reaction (PCR). Degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial MOMP genes were used in a PCR to amplify a DNA fragment of approximately...

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Identifiers

PMID: 1349899
DOI: 10.1128/jcm.30.5.1098-1104.1992
PMCID: PMC265232

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Source receipt: pubmed, retrieved 7 Sept 2026.

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