Article
Two-step polymerase chain reactions and restriction endonuclease analyses detect and differentiate ompA DNA of Chlamydia spp.
Journal of clinical microbiology - 1 May 1992
Kaltenboeck B, Kousoulas K G, Storz J
Abstract excerpt
Specific and sensitive amplification of major outer membrane protein (MOMP) gene (ompA) DNA sequences of Chlamydia species with various MOMP genotypes was achieved by a two-step polymerase chain reaction (PCR). Degenerate, inosine-containing oligonucleotide primers homologous to the 5' and 3' ends of the translated regions of all chlamydial MOMP genes were used in a PCR to amplify a DNA fragment of approximately...
Topics
- Animals
- Bacterial Outer Membrane Proteins
- Base Sequence
- Chlamydia
- DNA, Bacterial
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Restriction Mapping