Article
Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis.
Journal of clinical microbiology - 1 May 1993
Lan J, Walboomers J M, Roosendaal R, van Doornum G J, MacLaren D M, Meijer C J, van den Brule A J
Abstract excerpt
Detection and genotyping of Chlamydia trachomatis were optimized by using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis performed directly with crude cells of cervical scrapes. Different PCR pretreatment methods were evaluated on samples which were...
Topics
- Bacterial Typing Techniques
- Base Sequence
- Chlamydia Infections
- Chlamydia trachomatis
- DNA, Bacterial
- Female
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length