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Article

Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis.

Journal of clinical microbiology - 1 May 1993

Lan J, Walboomers J M, Roosendaal R, van Doornum G J, MacLaren D M, Meijer C J, van den Brule A J

Abstract excerpt

Detection and genotyping of Chlamydia trachomatis were optimized by using a polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis performed directly with crude cells of cervical scrapes. Different PCR pretreatment methods were evaluated on samples which were positive for C. trachomatis by cell culture. In comparison with DNA extraction and different proteolytic digestion...

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Identifiers

PMID: 8099080
DOI: 10.1128/jcm.31.5.1060-1065.1993
PMCID: PMC262880

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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