Article
A highly sensitive, multiplex broad-spectrum PCR-DNA-enzyme immunoassay and reverse hybridization assay for rapid detection and identification of Chlamydia trachomatis serovars.
The Journal of molecular diagnostics : JMD - 1 Nov 2007
Quint Koen D, van Doorn Leen-Jan, Kleter Bernhard, de Koning Maurits N C, van den Munckhof Henk A M, Morre Servaas A, ter Harmsel Bram, Weiderpass Elisabete, Harbers Gonneke, Melchers Willem J G, Quint Wim G V
Abstract excerpt
Chlamydia trachomatis (Ct) comprises distinct serogroups and serovars. The present study evaluates a novel Ct amplification, detection, and genotyping method (Ct-DT assay). The Ct-DT amplification step is a multiplex broad-spectrum PCR for the cryptic plasmid and the VD2-region of ompl. The Ct-DT detection step involves a DNA enzyme immunoassay (DEIA) using probes for serogroups (group B, C, and intermediate) and...
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