Article
Characterization of natural populations of Nitrobacter spp. using PCR/RFLP analysis of the ribosomal intergenic spacer.
Archives of microbiology - 1 Jan 1992
Navarro E, Simonet P, Normand P, Bardin R
Abstract excerpt
DNA sequences from the intergenic spacer (IGS) region of the ribosomal operon were amplified by the polymerase chain reaction (PCR) technique using two primers derived from 16S and 23S rRNA conserved sequences. The PCR products, cleaved by 4 base cutting restriction enzymes, were used to differen...
Topics
- Base Sequence
- DNA, Bacterial
- DNA, Ribosomal
- Genotype
- Molecular Sequence Data
- Nitrobacter
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- RNA, Ribosomal, 16S
- RNA, Ribosomal, 23S
- Restriction Mapping
- Soil Microbiology
- Water Microbiology