Article
Typing method for N2-fixing bacteria based on PCR-RFLP--application to the characterization of Frankia strains.
Molecular ecology - 1 Feb 1993
Jamann S, Fernandez M P, Normand P
Abstract excerpt
DNA sequences of an intergenic spacer (IGS) and parts of genes in the nif cluster were amplified by the polymerase chain reaction (PCR) using two primers derived from nifD- and nifK-conserved sequences. The PCR products were cleaved by ten 4-base cutting restriction enzymes and the restriction pa...
Topics
- Actinomycetales
- Bacterial Typing Techniques
- Base Sequence
- DNA Fingerprinting
- DNA Primers
- DNA, Bacterial
- DNA, Ribosomal
- Ecosystem
- Genes, Bacterial
- Genotype
- Klebsiella
- Molecular Sequence Data
- Nitrogen Fixation
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length