Article
An improved electrophoretic method for the determination of serum milk protein variants in Gyr-Holstein cows.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Jan 1992
Ramos P R, Urtado S L, Almeida M R, Bortolozzi J, Silva E T
Abstract excerpt
Milk serum proteins such as alpha-lactalbumin (ALA) and beta-lactoglobulin (BLG) present biochemical polymorphism which is under the control of codominant autosomal alleles. In the present report, we propose modifications of traditional electrophoretic techniques such as increasing the running gel concentration from 5 to 10% and the addition of 5 M urea to the stacking gel, which permitted the detection of two...
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