Article
Genetic transformation of Populus trichocarpa genotype Nisqually-1: a functional genomic tool for woody plants.
Plant & cell physiology - 1 Nov 2006
Song Jingyuan, Lu Shanfa, Chen Zenn-Zong, Lourenco Rodrigo, Chiang Vincent L
Abstract excerpt
We report here the Agrobacterium-mediated genetic transformation of Nisqually-1, a Populus trichocarpa genotype whose genome was recently sequenced. Several systems were established. Internodal stem segments from vigorously growing greenhouse plants are the explants most amenable to transformation. For the most efficient system, approximately 40% of the stem segments infected with pBI121-containing Agrobacterium...
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