Article
Scale-up and optimization of the low-temperature inducible cspA promoter system.
Biotechnology progress - 1 Jan 2000
Vasina J A, Peterson M S, Baneyx F
Abstract excerpt
The performance of the major Escherichia coli cold-shock promoter in directing the synthesis of recombinant proteins at low temperatures was investigated in batch fermentations using a plasmid-encoded transcriptional gene fusion between the cspA promoter region and the lacZ gene. Rapid synthesis...
Topics
- Bacterial Proteins
- Biotechnology
- Cold Temperature
- Escherichia coli
- Escherichia coli Proteins
- Fermentation
- Gene Expression
- Genes, Bacterial
- Glucuronidase
- Lac Operon
- Mutation
- Promoter Regions, Genetic
- RNA-Binding Proteins
- Recombinant Proteins
- Repressor Proteins
- Ribosomal Proteins
