Article
[Study of intermediate N using mutant forms of bacteriorhodopsin at Asp-96].
Biokhimiia (Moscow, Russia) - 1 Oct 1992
Danshina S V, Drachev L A, Kaulen A D, Korana Kh G, Marti T, Mogi T, Skulachev V I
Abstract excerpt
It has been found that the N(P, R)-type intermediate of the photocycle is formed in the Asp-96-->Asn mutant at acidic pH. Azide, which strongly activates the M decay in this mutant, allows the N intermediate to be shown also at neutral pH. Under these conditions mutant N decays in a pH-independent fashion. In the presence of azide, the H+ uptake by Asp-96-->Asn mutant bacteriorhodopsin follows the M decay,...
Topics
- Aspartic Acid
- Bacteriorhodopsins
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Protons