Article
Structures of aspartic acid-96 in the L and N intermediates of bacteriorhodopsin: analysis by Fourier transform infrared spectroscopy.
Biochemistry - 19 May 1992
Maeda A, Sasaki J, Shichida Y, Yoshizawa T, Chang M, Ni B, Needleman R, Lanyi J K
Abstract excerpt
The light-induced difference Fourier transform infrared spectrum between the L or N intermediate minus light-adapted bacteriorhodopsin (BR) was measured in order to examine the protonated states and the changes in the interactions of carboxylic acids of Asp-96 and Asp-115 in these intermediates. Vibrational bands due to the protonated and unprotonated carboxylic acid were identified by isotope shift and band...
Topics
- Acetates
- Acetic Acid
- Amino Acid Sequence
- Aspartic Acid
- Bacteriorhodopsins
- Carbon Isotopes
- Deuterium
- Fourier Analysis
- Hydrogen Bonding
- Mutation
- Protons