Article
Properties of recombinant cells capable of growing on serine without NhaB Na+/H+ antiporter in Escherichia coli.
Journal of bacteriology - 1 Nov 1992
Kayahara T, Thelen P, Ogawa W, Inaba K, Tsuda M, Goldberg E B, Tsuchiya T
Abstract excerpt
Escherichia coli HIT-1 has a mutation in the Na+/H+ antiporter gene, nhaB (P. Thelen, T. Tsuchiya, and E. B. Goldberg, J. Bacteriol. 173:6553-6557, 1991). This strain is not able to utilize serine as a carbon source (T. Ishikawa, H. Hama, M. Tsuda, and T. Tsuchiya, J. Biol. Chem. 262:7443-7446, 1987), because an active NhaB is required to maintain the electrochemical potential of Na+, which drives serine...
Topics
- Biological Transport
- Carbonyl Cyanide m-Chlorophenyl Hydrazone
- Carrier Proteins
- Cell Membrane
- Escherichia coli
- Genes, Bacterial
- Glycerol
- Hydrogen-Ion Concentration
- Kinetics
- Mutation
- Recombination, Genetic