Article
Lack of evidence for antisense suppression in the fungal plant pathogen Ustilago maydis.
Antisense & nucleic acid drug development - 1 Feb 1999
Keon J P, Owen J W, Hargreaves J A
Abstract excerpt
Modulation of the expression of the Ustilago maydis Pyr3 gene, encoding dihydroorotase (DHOase), through antisense RNA regulation has been explored. This was done by placing the gene in sense and antisense orientations under the control of an hsp70-like gene promoter in a high-copy number autonomously replicating expression vector. Cells transformed with the antisense vector contained similar levels of DHOase...
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