Lack of evidence for antisense suppression in the fungal plant pathogen Ustilago maydis.

doi:10.1089/oli.1.1999.9.101

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Article

Lack of evidence for antisense suppression in the fungal plant pathogen Ustilago maydis.

Antisense & nucleic acid drug development - 1 Feb 1999

Keon J P, Owen J W, Hargreaves J A

Abstract excerpt

Modulation of the expression of the Ustilago maydis Pyr3 gene, encoding dihydroorotase (DHOase), through antisense RNA regulation has been explored. This was done by placing the gene in sense and antisense orientations under the control of an hsp70-like gene promoter in a high-copy number autonomously replicating expression vector. Cells transformed with the antisense vector contained similar levels of DHOase...

Topics

Blotting, Northern
Blotting, Southern
Carboxin
Dihydroorotase
Fungal Proteins
Gene Expression
Gene Expression Regulation, Enzymologic
HSP70 Heat-Shock Proteins
Phenotype
Promoter Regions, Genetic
RNA, Antisense
RNA, Messenger
Transformation, Genetic
Uridine
Ustilago

Source

Open in PubMed

Identifiers

PMID: 10192295
DOI: 10.1089/oli.1.1999.9.101

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