A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

doi:10.1007/BF00281614

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Article

A gene cluster for amylovoran synthesis in Erwinia amylovora: characterization and relationship to cps genes in Erwinia stewartii.

Molecular & general genetics : MGG - 1 May 1993

Bernhard F, Coplin D L, Geider K

Abstract excerpt

A large ams gene cluster required for production of the acidic extracellular polysaccharide (EPS) amylovoran by the fire blight pathogen Erwinia amylovora was cloned. Tn5 mutagenesis and gene replacement were used to construct chromosomal ams mutants. Five complementation groups, essential for am...

Topics

Cloning, Molecular
DNA Transposable Elements
Erwinia
Gene Library
Genes, Bacterial
Genetic Complementation Test
Multigene Family
Mutagenesis
Phenotype
Plasmids
Polysaccharides, Bacterial
Restriction Mapping

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