Bacteriophage Mu as a genetic tool to study Erwinia amylovora pathogenicity and hypersensitive reaction on tobacco.

Abstract excerpt

Erwinia amylovora 1430 was shown to be sensitive to Mu G(-) particles. Infection resulted either in lytic development or in lysogenic derivatives with insertion of the Mu genome at many sites in the bacterial chromosome. We used the Mu d1Bx::Tn9 (lac Apr Cmr) derivative, called Mu dX, to identify mutants affected in pathogenicity and in their ability to induce a hypersensitive reaction (HR) on tobacco plants....

Topics

• Bacteriophage mu
• Blotting, Southern
• Chromosomes, Bacterial
• Erwinia
• Escherichia coli
• Genes, Viral
• Lysogeny
• Mutation
• Plants, Toxic
• Restriction Mapping
• Nicotiana