Article
Tn4556 and luciferase: synergistic tools for visualizing transcription in Streptomyces.
Gene - 15 Jun 1992
Sohaskey C D, Im H, Nelson A D, Schauer A T
Abstract excerpt
Progress in understanding genetic regulatory controls in the Actinomycetes has been rate limited by the properties of in vivo transcriptional probes. We have developed a set of plasmid- and transposon-based promoter-probe vectors that employ the Vibrio harveyi luciferase-encoding luxAB cassette as a reporter of transcription. The primary advantages of luciferase (Lux) over other reporter gene products are: (i)...
Topics
- DNA Transposable Elements
- Genetic Vectors
- Luciferases
- Mutation
- Promoter Regions, Genetic
- Streptomyces
- Transcription, Genetic