Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli.

doi:10.1128/jb.178.18.5382-5387.1996

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Article

Increased unsaturated fatty acid production associated with a suppressor of the fabA6(Ts) mutation in Escherichia coli.

Journal of bacteriology - 1 Sept 1996

Rock C O, Tsay J T, Heath R, Jackowski S

Abstract excerpt

Plasmids that corrected the temperature-sensitive unsaturated fatty acid auxotrophy of strain M6 [fabA6 (Ts)] were isolated from an Escherichia coli genomic library. Subcloning and physical mapping localized the new gene (called sfa for suppressor of fabA) at 1,070 kb on the E. coli chromosome. DNA sequencing revealed the presence of a 227-bp open reading frame which directed the synthesis of a peptide of...

Topics

Alleles
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Escherichia coli
Escherichia coli Proteins
Fatty Acid Synthase, Type II
Fatty Acids, Unsaturated
Gene Expression Regulation, Bacterial
Genes, Bacterial
Genes, Suppressor
Genomic Library
Hydro-Lyases

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Protein Biosynthesis

Restriction Mapping

Selection, Genetic

Source

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Identifiers

PMID: 8808925
DOI: 10.1128/jb.178.18.5382-5387.1996
PMCID: PMC178354

Provenance

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