Article
Molecular characterization of an Escherichia coli mutant with a temperature-sensitive malonyl coenzyme A-acyl carrier protein transacylase.
FEBS letters - 18 Jul 1994
Verwoert I I, Verhagen E F, van der Linden K H, Verbree E C, Nijkamp H J, Stuitje A R
Abstract excerpt
The temperature-sensitive malonyl CoA-ACP transacylase found in the Escherichia coli strain LA2-89, carrying the fabD89 allele, was shown to result from the presence of an amber mutation in the fabD gene, at codon position 257, in combination with the supE44 genotype of this strain. The truncated...
Topics
- Acyl-Carrier Protein S-Malonyltransferase
- Acyltransferases
- Alleles
- Base Sequence
- Codon
- Enzyme Stability
- Escherichia coli
- Escherichia coli Proteins
- Fatty Acid Synthase, Type II
- Fatty Acids
- Hot Temperature
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Temperature
- Tryptophan