Article
Overexpression of restructured pyruvate dehydrogenase complexes and site-directed mutagenesis of a potential active-site histidine residue.
The Biochemical journal - 15 Jul 1990
Russell G C, Guest J R
Abstract excerpt
The aceEF-lpd operon of Escherichia coli encodes the pyruvate dehydrogenase (E1p), dihydrolipoamide acetyltransferase (E2p) and dihydrolipoamide dehydrogenase (E3) components of the pyruvate dehydrogenase multienzyme complex (PDH complex). A thermoinducible expression system was developed to amplify a variety of genetically restructured PDH complexes, including those containing three, two, one and no lipoyl...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Codon
- Escherichia coli
- Gene Expression
- Histidine
- Molecular Sequence Data
- Mutation
- Oligonucleotides