Article
The yeast mitochondrial intron aI5 alpha: associated endonuclease activity and in vivo mobility.
Gene - 1 Apr 1992
Séraphin B, Faye G, Hatat D, Jacq C
Abstract excerpt
By analyzing crosses between yeast strains carrying different combinations of mitochondrial (mt) introns, we have shown that the aI5 alpha intron is mobile in vivo. Furthermore, we have observed that the mobility of intron aI5 alpha is affected by both the nuclear and mt genotypes. We have also detected a restriction endonuclease (ENase) activity that cleaves intronless mt genomes close to the aI5 alpha intron...
Topics
- Base Sequence
- Blotting, Southern
- Cell Nucleus
- Crosses, Genetic
- DNA Restriction Enzymes
- DNA, Fungal
- DNA, Mitochondrial
- Genome, Fungal
- Genotype
- Introns
- Molecular Sequence Data
- Oligodeoxyribonucleotides