Article
Retrotransposition of a yeast group II intron occurs by reverse splicing directly into ectopic DNA sites.
Proceedings of the National Academy of Sciences of the United States of America - 6 Nov 2001
Dickson L, Huang H R, Liu L, Matsuura M, Lambowitz A M, Perlman P S
Abstract excerpt
Group II introns, the presumed ancestors of nuclear pre-mRNA introns, are site-specific retroelements. In addition to "homing" to unoccupied sites in intronless alleles, group II introns transpose at low frequency to ectopic sites that resemble the normal homing site. Two general mechanisms have been proposed for group II intron transposition, one involving reverse splicing of the intron RNA directly into an...
Topics
- Base Sequence
- DNA Primers
- DNA, Fungal
- Introns
- Mutation
- Polymerase Chain Reaction
- RNA, Fungal
- Retroelements
- Saccharomyces cerevisiae