Article
Use of second-site homologous recombination to demonstrate that Epstein-Barr virus nuclear protein 3B is not important for lymphocyte infection or growth transformation in vitro.
Journal of virology - 1 May 1992
Tomkinson B, Kieff E
Abstract excerpt
Recombinant Epstein-Barr viruses with a stop codon inserted into the nuclear protein 3B (EBNA 3B) open reading frame were generated by second-site homologous recombination. These mutant viruses infected and growth transformed primary B lymphocytes, resulting in the establishment of lymphoblastoid...
Topics
- Antigens, Viral
- B-Lymphocytes
- Base Sequence
- Cell Division
- Cell Line
- Epstein-Barr Virus Nuclear Antigens
- Genome, Viral
- Herpesvirus 4, Human
- Humans
- Lymphocyte Activation
- Molecular Sequence Data
- Mutation
- Plasmids
- Recombination, Genetic
- Serial Passage
- Transcription, Genetic
- Virus Replication