Article
Culture of human osteoblasts on demineralised human bone. Possible means of graft enhancement.
The Journal of bone and joint surgery. British volume - 1 Mar 1992
Nolan P C, Nicholas R M, Mulholland B J, Mollan R A, Wilson D J
Abstract excerpt
We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on...
Topics
- Alkaline Phosphatase
- Bone and Bones
- Cells, Cultured
- Cyclic AMP
- Decalcification Technique
- Dinoprostone
- Dose-Response Relationship, Drug
- Female
- Graft Enhancement, Immunologic
- Humans
- Male
- Microscopy, Electron, Scanning
- Osteoblasts
- Phenotype