Article
Reaction of the nucleotide analogue 2-[(4-bromo-2,3-dioxobutyl)thio]adenosine 2',5'-bisphosphate at the coenzyme site of wild-type and mutant NADP(+)-specific glutamate dehydrogenases from Salmonella typhimurium.
Archives of biochemistry and biophysics - 1 Jan 1992
Haeffner-Gormley L, Chen Z D, Zalkin H, Colman R F
Abstract excerpt
Wild-type glutamate dehydrogenase (EC 1.4.1.4) from Salmonella typhimurium reacts at 25 degrees C in 0.1 M phosphate buffer, pH 7, with the nucleotide analogue 2-[(4-bromo-2,3-dioxobutyl)thio]-adenosine 2',5'-bisphosphate (2-BDB-TA 2',5'-DP) to give 78% inactivation. Protection against inactivati...
Topics
- Adenosine Diphosphate
- Affinity Labels
- Amino Acid Sequence
- Binding Sites
- Cross-Linking Reagents
- Enzyme Activation
- Glutamate Dehydrogenase
- Glutamate Dehydrogenase (NADP+)
- Hydrolysis
- Kinetics
- Molecular Sequence Data
- Mutation
- Peptide Mapping
- Salmonella typhimurium