Article
A high throughout assay for human papillomavirus genotypes with fluorescence polarization.
Chinese medical journal - 1 Aug 2003
Zhang Ju, Yan Xiaojun, Sun Jianzhong, Chen Zhongcan, Gao Yan'e, Bai Yujie, Liu Zhiguang
Abstract excerpt
OBJECTIVE: To develop a simple, cheap, quick, accurate and practical method for a high throughout genotypes assay of human papillomavirus (HPV) DNA. METHODS: Crude DNA was extracted by a simplified proteinase K digesting method. HPV common conservative primers: GP5+/6+ system was used to amplify HPV DNA in 127 samples of condylomata acuminatum (CA) and cervical scrapes by PCR, then the PCR product was assayed...
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