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Article

A high throughout assay for human papillomavirus genotypes with fluorescence polarization.

Chinese medical journal - 1 Aug 2003

Zhang Ju, Yan Xiaojun, Sun Jianzhong, Chen Zhongcan, Gao Yan'e, Bai Yujie, Liu Zhiguang

Abstract excerpt

OBJECTIVE: To develop a simple, cheap, quick, accurate and practical method for a high throughout genotypes assay of human papillomavirus (HPV) DNA. METHODS: Crude DNA was extracted by a simplified proteinase K digesting method. HPV common conservative primers: GP5+/6+ system was used to amplify HPV DNA in 127 samples of condylomata acuminatum (CA) and cervical scrapes by PCR, then the PCR product was assayed...

Topics

Base Sequence
DNA, Viral
Fluorescence Polarization
Genotype
Humans
Papillomaviridae
Papillomavirus Infections
Polymerase Chain Reaction
Tumor Virus Infections

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 12935397

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-12935397-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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