Article
Asymmetric GP5+/6+ PCR and hybridization with fluorescence polarization assay of 15 human papillomavirus genotypes in clinical samples.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology - 1 Feb 2009
Ju Zhang, Yane Gao, Ding Li, Guowu Yin, Yanhai Guo, Yonglan Liu
Abstract excerpt
BACKGROUND: The detection of a broad spectrum of HPV genotypes has not been used widely in cervical cancer screening due to technical difficulties and high costs. OBJECTIVES: To develop an asymmetric GP5+/6+ PCR assay and hybridization with a fluorescence polarization (FP) assay of 15 HPV genotypes. STUDY DESIGN: HPV genes in controls and samples were amplified by an asymmetric GP5+/6+ PCR. Fifteen HPV genotypic...
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