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In vitro oocyte culture and somatic cell nuclear transfer used to produce a live-born cloned goat.

Cloning and stem cells - 1 Jan 2003

Ohkoshi Katsuhiro, Takahashi Seiya, Koyama Shin-Ichiro, Akagi Satoshi, Adachi Noritaka, Furusawa Tadashi, Fujimoto Jun-Ichiro, Takeda Kumiko, Kubo Masanori, Izaike Yoshiaki, Tokunaga Tomoyuki

Abstract excerpt

The use of an in vitro culture system was examined for production of somatic cells suitable for nuclear transfer in the goat. Goat cumulus-oocyte complexes were incubated in tissue culture medium TCM-199 supplemented with 10% fetal bovine serum (FBS) for 20 h. In vitro matured (IVM) oocytes were enucleated and used as karyoplast recipients. Donor cells obtained from the anterior pituitary of an adult male were...

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PMID: 12930622
DOI: 10.1089/153623003322234704

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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