Article
Production of cloned goats after nuclear transfer using adult somatic cells.
Biology of reproduction - 1 Jan 2002
Keefer C L, Keyston R, Lazaris A, Bhatia B, Begin I, Bilodeau A S, Zhou F J, Kafidi N, Wang B, Baldassarre H, Karatzas C N
Abstract excerpt
The developmental potential of adult somatic nuclei after nuclear transfer (NT) into enucleated, in vitro-matured oocytes was evaluated in a dwarf breed of goat (BELE: Breed Early Lactate Early). Somatic donor cells were obtained from two different sources: 1) adult granulosa cells (GCs) and 2) fetal fibroblasts. Primary GCs were obtained from follicular aspirants after laparoscopic oocyte pick-up (LOPU) and were...
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