Production of cloned goats after nuclear transfer using adult somatic cells.

doi:10.1095/biolreprod66.1.199

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Article

Production of cloned goats after nuclear transfer using adult somatic cells.

Biology of reproduction - 1 Jan 2002

Keefer C L, Keyston R, Lazaris A, Bhatia B, Begin I, Bilodeau A S, Zhou F J, Kafidi N, Wang B, Baldassarre H, Karatzas C N

Abstract excerpt

The developmental potential of adult somatic nuclei after nuclear transfer (NT) into enucleated, in vitro-matured oocytes was evaluated in a dwarf breed of goat (BELE: Breed Early Lactate Early). Somatic donor cells were obtained from two different sources: 1) adult granulosa cells (GCs) and 2) fetal fibroblasts. Primary GCs were obtained from follicular aspirants after laparoscopic oocyte pick-up (LOPU) and were...

Topics

Animals
Cell Fusion
Cell Nucleus
Cloning, Organism
Embryo Transfer
Female
Fertilization in Vitro
Fibroblasts
Genotype
Goats
Granulosa Cells

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Laparoscopy

Nuclear Transfer Techniques

Oocytes

Polymorphism, Single-Stranded Conformational

Pregnancy

Reverse Transcriptase Polymerase Chain Reaction

Source

Open in PubMed

Identifiers

PMID: 11751283
DOI: 10.1095/biolreprod66.1.199

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