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Development of an Optimized Zona-Free Method of Somatic Cell Nuclear Transfer in the Goat

1 Apr 2011

Abstract excerpt

The purpose of this study was to develop an improved zona-free method of goat somatic cell nuclear transfer (SCNT) that has both ease of operation and efficiency. The main steps involved were: (1) optimization of in vitro oocyte maturation, (2) parthenogenetic activation of zona-free oocytes, (3) SCNT of zona-free anaphase II-telophase II (AII-TII) oocytes that subverted the need for long term UV-exposure of the...

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PMID: 21473692
DOI: 10.1089/cell.2010.0083

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