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Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes.

Biology of reproduction - 1 Mar 2001

Keefer C L, Baldassarre H, Keyston R, Wang B, Bhatia B, Bilodeau A S, Zhou J F, Leduc M, Downey B R, Lazaris A, Karatzas C N

Abstract excerpt

The developmental potential of caprine fetal fibroblast nuclei after in vitro transfection and nuclear transfer (NT) into enucleated, in vitro-matured oocytes was evaluated. Fetal fibroblasts were isolated from Day 27 to Day 30 fetuses from a dwarf breed of goat (BELE: breed early lactate early). Cells were transfected with constructs containing the enhanced green fluorescent protein (eGFP) and neomycin...

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PMID: 11207200
DOI: 10.1095/biolreprod64.3.849

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