Article
Cloning missy: obtaining multiple offspring of a specific canine genotype by somatic cell nuclear transfer.
Cloning and stem cells - 1 Mar 2009
Hossein Mohammad Shamim, Jeong Yeon Woo, Park Sun Woo, Kim Joung Joo, Lee Eugine, Ko Kyeong Hee, Kim Huen Suk, Kim Yeun Wook, Hyun Sang Hwan, Shin Taeyoung, Hawthorne Lou, Hwang Woo Suk
Abstract excerpt
The present study was undertaken to evaluate two activation methods for somatic cell nuclear transfer (SCNT), namely, fusion and simultaneous activation (FSA, fusion medium contains calcium), versus fusion followed by chemical activation (F+CA, fusion medium does not contain calcium), and to evaluate the effects of parity of recipient dogs on the success of SCNT. Oocytes retrieved from outbred dogs were...
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