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Somatic Cell Cloning in Buffalo ( <i>Bubalus bubalis</i> ): Effects of Interspecies Cytoplasmic Recipients and Activation Procedures

1 Sept 2001

Abstract excerpt

Successful nuclear transfer (NT) of somatic cell nuclei from various mammalian species to enucleated bovine oocytes provides a universal cytoplast for NT in endangered or extinct species. Buffalo fetal fibroblasts were isolated from a day 40 fetus and were synchronized in presumptive G(0) by serum deprivation. Buffalo and bovine oocytes from abattoir ovaries were matured in vitro and enucleated at 22 h. In the...

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PMID: 11945219
DOI: 10.1089/153623001753205052

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