Article
Cold-active esterase from Psychrobacter sp. Ant300: gene cloning, characterization, and the effects of Gly-->Pro substitution near the active site on its catalytic activity and stability.
Biochimica et biophysica acta - 14 Jan 2004
Kulakova Ljudmila, Galkin Andrey, Nakayama Toru, Nishino Tokuzo, Esaki Nobuyoshi
Abstract excerpt
The gene encoding an esterase (PsyEst) of Psychrobacter sp. Ant300, a psychrophilic bacterium isolated from Antarctic soil, was cloned, sequenced, and expressed in Escherichia coli. PsyEst, which is a member of hormone-sensitive lipase (HSL) group of the lipase/esterase family, is a cold-active,...
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