Article
Site-directed mutagenesis of the active site of diacylglycerol kinase alpha: calcium and phosphatidylserine stimulate enzyme activity via distinct mechanisms.
The Biochemical journal - 1 Nov 2003
Abe Takahiro, Lu Xiaolan, Jiang Ying, Boccone Clark E, Qian Shaomin, Vattem Krishna M, Wek Ronald C, Walsh James P
Abstract excerpt
Diacylglycerol kinases (DAGKs) catalyse ATP-dependent phosphorylation of sn-1,2-diacylglycerol that arises during stimulated phosphatidylinositol turnover. DAGKa is activated in vitro by Ca2+ and by acidic phospholipids. The regulatory region of DAGKa includes an N-terminal RVH motif and EF hands that mediate Ca2+-dependent activation. DAGKa also contains tandem C1 protein kinase C homology domains. We utilized...
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