Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces.

Journal of medical microbiology - 1 Aug 2003

Amar Corinne F L, Dear Paul H, McLauchlin Jim

Abstract excerpt

A nested PCR assay (TPILC-PCR) was developed to detect and distinguish between Giardia duodenalis assemblages A and B from human faeces by analysis of the triose phosphate isomerase gene (tpi). The assay comprised an initial multiplexed block-based amplification. This was followed by two separate real-time PCR assays specific for assemblages A and B using a LightCycler and SYBR Green I to identify PCR products by...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Feces
Share to Genotype
Share to Giardia

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Giardiasis

Share to Parasitic Infections and Diagnostics

Share to Polymerase Chain Reaction

Share to Polymorphism, Restriction Fragment Length

Share to Sensitivity and Specificity

Source

Identifiers

PMID: 12867562
DOI: 10.1099/jmm.0.05193-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces.PMID 12867562Read ↗

Real-time PCR/RFLP assay to detect Giardia intestinalis genotypes in human isolates with diarrhea in Egypt.Semantic proximity Read ↗

Sensitive PCR-Restriction Fragment Length Polymorphism Assay for Detection and Genotyping ofGiardia duodenalisin Human FecesSemantic proximity Read ↗

Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples.Semantic proximity Read ↗

Real-time PCR assay for detection and genotype differentiation of Giardia lamblia in stool specimens.Semantic proximity Read ↗

A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates.Semantic proximity Read ↗

Comparative Evaluation of Real-Time Screening PCR Assays for Giardia duodenalis and of Assays Discriminating the Assemblages A and BSemantic proximity Read ↗

Giardia duodenalis typing from stools: a comparison of three approaches to extracting DNA, and validation of a probe-based real-time PCR typing assaySemantic proximity Read ↗

A modified PCR-RFLP method to determine genetic diversity of Giardia lamblia human isolates based on triosephosphate isomerase (TPI) gene.Semantic proximity Read ↗

Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.Semantic proximity Read ↗

Development of HRM real-time PCR for assemblage characterization of Giardia lamblia.Semantic proximity Read ↗

Sequence analysis of the beta-giardin gene and development of a polymerase chain reaction-restriction fragment length polymorphism assay to genotype Giardia duodenalis cysts from human faecal samples.Semantic proximity Read ↗

PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.