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A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates.

Diagnostic microbiology and infectious disease - 1 Dec 2024

Ghosal Ajanta, Sardar Sanjib K, Haldar Tapas, Prasad Akash, Das Koushik, Kobayashi Seiki, Saito-Nakano Yumiko, Dutta Shanta, Nozaki Tomoyoshi, Ganguly Sandipan

Abstract excerpt

Giardia duodenalis is a common cause of diarrheal illness in regions with limited resources. The demand for rapid and cost-effective detection and genotyping methods in large-scale epidemiological studies and clinical diagnostics is imperative. Hence, we developed a multiplex PCR-RFLP technique targeting the tpi gene of G. duodenalis. The assay successfully screened G. duodenalis positive clinical samples (6.33...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 39383635
DOI: 10.1016/j.diagmicrobio.2024.116548

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