Article
PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.
Parasitology research - 1 May 2016
Tan Liping, Wu Sheng, Abdullahi Auwalu Yusuf, Yu Xinggang, Hu Wei, Song Meiran, Shi Xianli, Li Guoqing
Abstract excerpt
Giardia duodenalis is a zoonotic protozoan that parasitizes the upper small intestine of human and many mammals including dogs. To develop a restriction fragment length polymorphism (RFLP) method for typing zoonotic (A, B) and host-specific (C, D) assemblages of G. duodenalis from dog, β-giardin gene was amplified with design primer pairs B3 and B4. The PCR products were digested with restriction enzyme Afa I and...
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