Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Giardia duodenalis typing from stools: a comparison of three approaches to extracting DNA, and validation of a probe-based real-time PCR typing assay

2 Oct 2013

Abstract excerpt

A weighted, multi-attribute approach was used to compare three methods for direct extraction of Giardia duodenalis DNA from 15 microscopy-positive stools: (1) a QIAamp spin-column method for stools including a 10 min incubation at 95 °C, (2) method 1 preceded by five freeze-thaw cycles and (3) bead beating with guanidine thiocyanate using a FastPrep-28 machine followed by liquid-phase silica purification of DNA....

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amoebic Infections and Treatments
Share to Parasitic Infections and Diagnostics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 24085818
DOI: 10.1099/jmm.0.066050-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Giardia duodenalis typing from stools: a comparison of three approaches to extracting DNA, and validation of a probe-based real-time PCR typing assayPMID 24085818Read ↗

A rapid economical multiplex PCR-RFLP method for molecular detection and genotyping of Giardia duodenalis clinical isolates.Semantic proximity Read ↗

Comparative Evaluation of Real-Time Screening PCR Assays for Giardia duodenalis and of Assays Discriminating the Assemblages A and BSemantic proximity Read ↗

Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces.Semantic proximity Read ↗

Giardia duodenalis: Detection by Quantitative Real-Time PCR and Molecular Diversity.Semantic proximity Read ↗

Suitability of current typing procedures to identify epidemiologically linked human Giardia duodenalis isolatesSemantic proximity Read ↗

Microscopy detection and molecular characterisation of Giardia duodenalis infection in outpatients seeking medical care in Egypt.Semantic proximity Read ↗

Zoonotic risk of transmission of Giardia duodenalis from water resources; Worldwide molecular and network analyses.Semantic proximity Read ↗

Evaluation of five commercial methods for the extraction and purification of DNA from human faecal samples for downstream molecular detection of the enteric protozoan parasites Cryptosporidium spp., Giardia duodenalis, and Entamoeba sppSemantic proximity Read ↗

Genotyping of Giardia duodenalis cysts by new real-time PCR assays for detection of mixed infections in human samples.Semantic proximity Read ↗

Diversity of domestic and imported assemblages of Giardia in Norway and multi-locus sequence typing of domestic assemblage A isolates.Semantic proximity Read ↗

LNA probes in a real-time TaqMan PCR assay for genotyping of Giardia duodenalis in wastewaters.Semantic proximity Read ↗

A modified PCR-RFLP method to determine genetic diversity of Giardia lamblia human isolates based on triosephosphate isomerase (TPI) gene.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.