Article
Dynamics of a transgene expression in acute rat brain slices transfected with adenoviral vectors.
Experimental physiology - 1 Jul 2003
Stokes C E L, Murphy D, Paton J F R, Kasparov S
Abstract excerpt
We present a quantitative account of the expression dynamics of a transgene (enhanced green fluorescent protein, EGFP) in acute brain slices transfected with an adenoviral vector (AVV) under control of the human cytomegalovirus (HCMV) promoter. Micromolar concentrations of EGFP could be detected in brainstem and hippocampal slices as early as 7 h after in vitro transfection with a viral titre of 4.4 x 10(9)...
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