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Article

Allele-specific Holliday junction formation: a new mechanism of allelic discrimination for SNP scoring.

Genome research - 1 Jul 2003

Yang Qinghong, Lishanski Alla, Yang Wendy, Hatcher Sandra, Seet Henrietta, Gregg Jeffrey P

Abstract excerpt

We report here a new mechanism for allelic discrimination--allele-specific Holliday Junction formation. The Holliday Junction (HJ) is a unique DNA structure that can be formed in a sequence-nonspecific manner by routine PCR. To cause the PCR-based HJ formation to occur in an allele-specific manner, the PCR primers are manipulated such that an extra mismatch next to a SNP of interest is introduced between a target...

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PMID: 12840050
DOI: 10.1101/gr.997703
PMCID: PMC403749

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