Article
Overexpression of the cochaperone CHIP enhances Hsp70-dependent folding activity in mammalian cells.
Molecular and cellular biology - 1 Jul 2003
Kampinga Harm H, Kanon Bart, Salomons Florian A, Kabakov Alexander E, Patterson Cam
Abstract excerpt
CHIP is a cochaperone of Hsp70 that inhibits Hsp70-dependent refolding in vitro. However, the effect of altered expression of CHIP on the fate of unfolded proteins in mammalian cells has not been determined. Surprisingly, we found that overexpression of CHIP in fibroblasts increased the refolding of proteins after thermal denaturation. This effect was insensitive to geldanamycin, an Hsp90 inhibitor, and required...
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