Superoxide dismutase folding/unfolding pathway: role of the metal ions in modulating structural and dynamical features.

Abstract excerpt

The unfolding and refolding of a monomeric mutant of copper-zinc superoxide dismutase was investigated by NMR spectroscopy in the copper-reduced form and by using guanidinium chloride as denaturing agent. It is found that the protein gives rise to a series of intermediates at low guanidinium concentration and to a globular unfolded state at a guanidinium concentration higher than 3.5 M, which closely resembles a...

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