Article
Enhanced secretion of heterologous cyclodextrin glycosyltransferase by a mutant of Bacillus licheniformis defective in the D-alanylation of teichoic acids.
Letters in applied microbiology - 1 Jan 2003
Craynest M, Jørgensen S, Sarvas M, Kontinen V P
Abstract excerpt
AIMS: To examine whether inactivation of the dlt operon and increased charge density of the wall enhances secretion of heterologous proteins in industrial strains of Bacillus licheniformis. METHODS AND RESULTS: The dltA gene of B. licheniformis was cloned, sequenced and mutated by inserting a chloramphenicol acetyl transferase (cat) gene cassette. The mutation facilitated growth in the late exponential growth...
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