Article
Identification of in vivo enzyme activities in the cometabolism of glucose and acetate by Saccharomyces cerevisiae by using 13C-labeled substrates.
Eukaryotic cell - 1 Jun 2003
dos Santos Margarida Moreira, Gombert Andreas Karoly, Christensen Bjarke, Olsson Lisbeth, Nielsen Jens
Abstract excerpt
A detailed characterization of the central metabolic network of Saccharomyces cerevisiae CEN.PK 113-7D was carried out during cometabolism of different mixtures of glucose and acetate, using aerobic C-limited chemostats in which one of these two substrates was labeled with (13)C. To confirm the role of malic enzyme, an isogenic strain with the corresponding gene deleted was grown under the same conditions. The...
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