Article
Binary transformation systems based on 'shooter' mutants of Agrobacterium tumefaciens: a simple, efficient and universal gene transfer technology that permits marker gene elimination.
Plant cell reports - 1 Apr 2003
Mihálka V, Balázs E, Nagy I
Abstract excerpt
A simple transformation procedure with a positive selection scheme using the expression of the isopentenyl transferase ( ipt) gene of transfer DNA (T-DNA) 'shooter' mutants of Agrobacterium tumefaciens was elaborated. After comparing several 'shooter' mutants we found that particular strains frequently produced phenotypically normal shoots after co-culturing with tobacco leaf explants. Shoots selected for normal...
Topics
- Agrobacterium tumefaciens
- Capsicum
- Cinnamates
- Cotyledon
- Cucumis melo
- Culture Techniques
- DNA, Bacterial
- Drug Resistance, Bacterial
- Gene Transfer Techniques
- Genetic Markers
- Genetic Vectors
- Hygromycin B
- Kanamycin