Article
Investigation into the role of apolipoprotein B gene 8344C/T variant on plasma cholesterol levels by allele-specific PCR amplification.
Annali dell'Istituto superiore di sanita - 1 Jan 2002
Cantafora Alfredo, Bertolini Stefano, Blotta Ida, Rivabene Roberto, Pisciotta Livia, Langheim Silvia, Calandra Sebastiano
Abstract excerpt
The 8344C/T polymorphism of the apoB gene was genotyped by an original modification of PCR allele-specific amplification consisting in a single amplification reaction double-primed by two opposite allele-specific oligonucleotides nested in a larger amplified fragment. This method was used to genotype 200 randomly selected healthy individuals (113 males, 87 females). The frequency of the rare allele in this random...
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