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Article

Affinity purification of ribosomes with a lethal G2655C mutation in 23 S rRNA that affects the translocation.

The Journal of biological chemistry - 11 Jul 2003

Leonov Andrei A, Sergiev Petr V, Bogdanov Alexey A, Brimacombe Richard, Dontsova Olga A

Abstract excerpt

A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and subsequent affinity chromatography. It was tested...

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Identifiers

PMID: 12730236
DOI: 10.1074/jbc.M302873200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026. Prepared record publication-12730236-2026-09-04.2. Manifest publication-graph-cohort-2026-09-04.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 4 Sept 2026.

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