Article
Optimisation of interdelta analysis for Saccharomyces cerevisiae strain characterisation.
FEMS microbiology letters - 25 Apr 2003
Legras Jean-Luc, Karst Francis
Abstract excerpt
A new primer pair (delta12-delta21) for polymerase chain reaction-based yeast typing was designed using the yeast genome sequence. The specificity of this primer pair was checked by the comparison of the electrophoresis pattern with a virtual profile calculated from Blast data. The analysis of 53 commercial and laboratory Saccharomyces cerevisiae yeast strains showed a clear improvement of interdelta analysis...
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